American Journal of Food Science and Nutrition (AJFSN)
Detoxification effect of Bifidobacterium bifidum against aflatoxin M1 in weaning baby food
(This article belongs to Vol - 02, Issue - 01)
Aflatoxin M1 (AFM1) concerns potent hazard in baby food for infant and young children. Bacterial detoxification is a promising method to reduce mycotoxins in food matrix. Bifidobacterium bifidum investigate the binding capacity towards AFM1 in phosphate buffered saline (PBS) and prepared baby food. The binding ability was evaluated regarding to bacterial population (107, 108 and 109 cfu/g), incubation intervals (0, 6, 12 and 24 hrs), viable and heat treated cells against AFM1. The unbound AFM1 was quantitative analyzed by Competitive Direct ELISA method. The experimental use of several concentrations of B. bifidum showed gradual AFM1 decrease forming binding AFM1-bacteria complex by incubation time. The AFM1 reduction percent/24hrs of B. bifidum cells ranged from 34.15 to 56.66% in PBS and from 35.77 to 64.10% in baby food samples. High bacterial concentration, nonviable bacteria and increase storage intervals are factors had significant (p<0.05) AFM1-bacteria binding ability to reduce AFM1 in spiked baby food samples contained 1x109 cfu/g of nonviable bacterial cells to be 17.72±0.68 ng/kg. The high bacterial concentration of nonviable cells show significant high detoxification effect in spiked samples, when compared with PBS (p<0.05) and may comply with the acceptable limits in the Egyptian standards. It could be concluded that, the bacterial population, heat-treated cells and time of incubation have a positive binding effect toward AFM1 in baby food.